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Flow-And-Shoot Technology (FAST)

 

Flow And Shoot Technology (FAST) integrates the existing RARAF microbeam technology with microfluidics tools. Instead of irradiating cells attached to a thin film, the cells are confined to a channel thinner than the range of the particles and as wide as the field of view of the imaging and beam targeting system. As the cells flow through the channel, they are continuously tracked and targeted using the point & shoot system, as shown in the movies below.

Tracking of 5 micron fluorescent beads flowing in a channel.

Tracking of GFP labeled CRL4025 cells, the yellow + denote the true current centroid of the cell. The cyan x denotes the cell’s position as predicted by the FAST tracking software.    


This real time tracking allows microbeam irradiation of lymphocytes and other cells that cannot be attached to a surface. It also allows, coupling the microbeam to microfluidic devices for cell sorting and analysis.

 




tel: (914) 591-9244
fax: (914) 591-9405
Radiological Research Accelerator Facility Nevis Laboratories
P.O. Box 21, 136 S. Broadway, Irvington, N.Y. 10533